ClustalX 2.1 was used for sequence alignments. The sequences were downloaded from the UniProt and ToxoDB websites. The access numbers for the corresponding proteins are as follows: (TgPFK1,ToxoDB gene ID: TGGT1_240890; ChPFK, Cryptosporidium hominis PFK, UniProtKB ID: A0A0S4TBK3_CRYHO; TbPFK, Trypanosoma brucei brucei PFK, UniProtKB ID: PFKA_TRYBB; LdPFK, Leishmania donovani PFK, UniProtKB ID: PFKA_LEIDO; CePFK, Caenorhabditis elegans PFK, UniProtKB ID: PFKA1_CAEEL; EhPFK, Entamoeba histolytica PFK, UniProtKB ID: PFKA_ENTHI; EcPFK, Escherichia coli (strain K12) PFK, UniProtKB ID: PFKA_ECOLI; AtPFK, Arabidopsis thaliana PFK UniProtKB ID: PFKA3_ARATH; HsPFK, Homo sapiens PFK, UniProtKB ID: PFKAP_HUMAN; SsPFK, Sus scrofa PFK, UniProtKB ID: PFKAM_PIG); ScPFK, Saccharomyces cerevisiae PFK (strain ATCC 204508/S288c), UniProtKB ID: PFKA1_YEAST; SaPFK, Staphylococcus aureus PFK, UniProtKB ID: PFKA_STAA8; EaPFK, Eimeria acervulina PFK, UniProtKB ID: U6G9T9_EIMAC; TaPFK, Triticum aestivum PFK, UniProtKB ID: A0A1D5UP90_WHEAT; TgPFK2, Toxoplasma gondii phosphofructokinase PFKII, ToxoDB gene ID:TGGT1_226960; ChPFP, Cryptosporidium hominis PFP, UniProtKB ID: A0A0S4TCQ1_CRYHO; EhPFP, Entamoeba histolytica PFP, UniProtKB ID: PFP_ENTHI; EaPFP, Eimeria acervulina PFP, UniProtKB ID: U6GFF7_EIMAC; Ath PFP, Arabidopsis thaliana PFP, UniProtKB ID: PFPB1_ARATH; TaPFP, Triticum aestivum PFP, UniProtKB ID: A0A1D6D1Q3_WHEAT; and StPFP, Spirochaeta thermophila PFP, UniProtKB ID: PFP_SPITD.
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